A simultaneous two-colour detection method of human IgG- and IgE-reactive proteins from lactic acid bacteria
- Source:
- Journal of microbiological methods 2016 v.126 pp. 72-75
- ISSN:
- 0167-7012
- Subject:
- Lactobacillus, Western blotting, antibodies, bacterial proteins, humans, immunoglobulin E, immunoglobulin G, lactic acid bacteria, patients
- Abstract:
- Whole cell extracts of two Lactobacillus strains were tested with primary antibodies from two pooled sera from allergic patients. Fluorescently labelled anti-human IgG and anti-human IgE secondary antibodies applied in Western blotting, together with an appropriate image acquisition protocol facilitated imagining bacterial proteins that reacted with human IgG and IgE.
- Agid:
- 5297038
- https://doi.org/10.1016/j.mimet.2016.05.007