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A simultaneous two-colour detection method of human IgG- and IgE-reactive proteins from lactic acid bacteria
- Markiewicz, Lidia Hanna, Szymkiewicz, Agata, Szyc, Anna, Wróblewska, Barbara
- Journal of microbiological methods 2016 v.126 pp. 72-75
- Lactobacillus, Western blotting, antibodies, bacterial proteins, humans, immunoglobulin E, immunoglobulin G, lactic acid bacteria, patients
- Whole cell extracts of two Lactobacillus strains were tested with primary antibodies from two pooled sera from allergic patients. Fluorescently labelled anti-human IgG and anti-human IgE secondary antibodies applied in Western blotting, together with an appropriate image acquisition protocol facilitated imagining bacterial proteins that reacted with human IgG and IgE.