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Immunoglobulin gene expression in umbilical cord blood-derived CD34+ hematopoietic stem/progenitor cells

Author:
Liu, Jingfang, Xia, Miaoran, Wang, Pingzhang, Wang, Chong, Geng, Zihan, Cameron Yin, C., Zhang, Chi, Qiu, Xiaoyan
Source:
Gene 2016 v.575 no.1 pp. 108-117
ISSN:
0378-1119
Subject:
blood, databases, fluorescent antibody technique, gene expression, immunoglobulin A, immunoglobulin M, immunoglobulin genes, microarray technology, stem cells, umbilical cord
Abstract:
Recently, immunoglobulin (Ig) expression was reported in a variety of non-B lineage cells, including myeloid cells. We assessed whether hematopoietic stem/progenitor cells (HSC/HPCs) can express Ig. With Gene Expression Omnibus (GEO) microarray database analysis, we found that IGHM was expressed with the highest frequency and level in umbilical cord blood CD34⁺ HSC/HPCs, followed by IGK@, IGHE, IGHD, IGHG1, and IGHA1, while IGL@ was nearly not expressed. Ig expression was further confirmed by molecular experiments and immunofluorescence. Moreover, HSC/HPCs-derived Ig displayed restricted/biased usages and VHDJH rearrangement patterns. These results suggest that Igs, especially IgM, may have a role in CD34⁺ HSC/HPCs function.
Agid:
5298211