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The antibacterial activity and action mechanism of emodin from Polygonum cuspidatum against Haemophilus parasuis in vitro

Author:
Li, Li, Song, Xu, Yin, Zhongqiong, Jia, Renyong, Li, Zhengwen, Zhou, Xun, Zou, Yuanfeng, Li, Lixia, Yin, Lizi, Yue, Guizhou, Ye, Gang, Lv, Cheng, Shi, Wenjing, Fu, Yuping
Source:
Microbiological research 2016 v.186-187 pp. 139-145
ISSN:
0944-5013
Subject:
Haemophilus parasuis, Reynoutria japonica, antibacterial properties, antibiotics, cell membranes, cell walls, disease outbreaks, emodin, financial economics, flow cytometry, fluorescence, mechanism of action, membrane permeability, membrane proteins, minimum inhibitory concentration, plasmolysis, pork industry, transmission electron microscopy
Abstract:
Haemophilus parasuis is the causative agent of Glässer’s disease, which leads to serious economic loss to the swine industry. Although antibiotics are widely used to control infections, outbreaks of this disease repeatedly happen. In this study, emodin from Polygonum cuspidatum showed potent inhibitory effect against H. parasuis. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values of emodin were 32 and 64μg/mL, respectively. The antibacterial kinetic curves indicated the antibacterial activity of emodin was in a concentration-dependent manner. Cell membrane permeability and flow cytometry assays proved that emodin could destroy cell membrane integrity and increase membrane permeability, and fluorescence spectra assay indicated emodin has influenced conformation of membrane protein. Under transmission electron microscopy, serious lesions of H. parasuis exposed to emodin (64μg/mL) were found, including irregular cell shape, plasmolysis, ruptured cell wall and membrane and cytoplasmic vacuolation. These results suggested that emodin could be used as candidate for treating Glässer’s disease.
Agid:
5301955