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A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery

Author:
Karim, Ashty S., Jewett, Michael C.
Source:
Metabolic engineering 2016 v.36 pp. 116-126
ISSN:
1096-7176
Subject:
DNA, Escherichia coli, biochemical pathways, biosynthesis, butanol, cell-free protein synthesis, enzymes, metabolic engineering, models
Abstract:
Speeding up design-build-test (DBT) cycles is a fundamental challenge facing biochemical engineering. To address this challenge, we report a new cell-free protein synthesis driven metabolic engineering (CFPS-ME) framework for rapid biosynthetic pathway prototyping. In our framework, cell-free cocktails for synthesizing target small molecules are assembled in a mix-and-match fashion from crude cell lysates either containing selectively enriched pathway enzymes from heterologous overexpression or directly producing pathway enzymes in lysates by CFPS. As a model, we apply our approach to n-butanol biosynthesis showing that Escherichia coli lysates support a highly active 17-step CoA-dependent n-butanol pathway in vitro. The elevated degree of flexibility in the cell-free environment allows us to manipulate physiochemical conditions, access enzymatic nodes, discover new enzymes, and prototype enzyme sets with linear DNA templates to study pathway performance. We anticipate that CFPS-ME will facilitate efforts to define, manipulate, and understand metabolic pathways for accelerated DBT cycles without the need to reengineer organisms.
Agid:
5302621