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Real-Time Bioluminescence Imaging of Nitroreductase in Mouse Model

Author:
Feng, Ping, Zhang, Huateng, Deng, Quankun, Liu, Wei, Yang, Linghui, Li, Guobo, Chen, Guo, Du, Lupei, Ke, Bowen, Li, Minyong
Source:
Analytical chemistry 2016 v.88 no.11 pp. 5610-5614
ISSN:
1520-6882
Subject:
animal models, animals, bioluminescence, image analysis, neoplasms
Abstract:
Nitroreductase (NTR) is an endogenous reductase overexpressed in hypoxic tumors; however, its precise detection in living cells and animals remains a considerable challenge. Herein, we developed three reaction-based probes and a related bioluminescence assay for the real-time NTR detection. The high sensitivity and selectivity of probe 3, combined with its remarkable potential of bioluminescence imaging, affords a valuable approach for in vivo imaging of NTR in a tumor model mouse.
Agid:
5304089