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A Fluorescent Chemodosimeter for Live-Cell Monitoring of Aqueous Sulfides

Wang, Shiguo, Xu, Suying, Hu, Gaofei, Bai, Xilin, James, Tony D., Wang, Leyu
Analytical chemistry 2016 v.88 no.2 pp. 1434-1439
detection limit, fluorescence, fluorescent dyes, image analysis, mass spectrometry, monitoring, nuclear magnetic resonance spectroscopy, sulfides, sulfur, thiols
Aqueous sulfides are emerging signaling agents implicated in various pathological and physiological processes. The development of sensitive and selective methods for the sensing of these sulfides is therefore very important. Herein, we report that the as-synthesized 1-oxo-1H-phenalene-2,3-dicarbonitrile (OPD) compound provides promising fluorescent properties and unique reactive properties toward aqueous sulfides. It was found that OPD showed high selectivity and sensitivity toward Na₂S over thiols and other inorganic sulfur compounds through a sulfide involved reaction which was confirmed by high-resolution mass spectroscopy (HRMS) and nuclear magnetic resonance (NMR) results. The fluorescence intensity increases linearly with sulfide concentration in the range of 1.0–30 μM with a limit of detection of 52 nM. This novel fluorescent probe was further exploited for the fluorescence imaging sensing of aqueous sulfide in HeLa cells.