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Simultaneous Photoelectrochemical Immunoassay of Dual Cardiac Markers Using Specific Enzyme Tags: A Proof of Principle for Multiplexed Bioanalysis

Zhang, Nan, Ma, Zheng-Yuan, Ruan, Yi-Fan, Zhao, Wei-Wei, Xu, Jing-Juan, Chen, Hong-Yuan
Analytical chemistry 2016 v.88 no.4 pp. 1990-1994
acetylcholinesterase, alkaline phosphatase, ascorbic acid, electrodes, hydrolysis, immunoassays, nanotubes, quantum dots, titanium dioxide
In this Letter, on the basis of the CdS quantum dots functionalized TiO₂ nanotubes electrode, we proposed a simultaneous photoelectrochemical (PEC) immunoassay of dual cardiac markers using specific enzyme tags of alkaline phosphatase (ALP) and acetylcholine esterase (AChE). ALP and AChE were integrated into the PEC system through the sandwich immunobinding and could specifically catalyze the hydrolysis of ascorbic acid 2-phosphate (AAP) or the acetylthiocholine (ATC) to in situ generate ascorbic acid (AA) or thiocholine (TC) for sacrificial electron donating. These two enzymes were thus used to differentiate the signals of two cardiac targets in connection with the sandwich immunorecognition and PEC responses to the corresponding electron donors. This strategy demonstrates a proof of principle for the successful integration of dual enzyme tags with PEC immunoassay that can potentially provide a general format for multiplexed PEC bioanalysis.