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HPLC-MS with Glass Chips Featuring Monolithically Integrated Electrospray Emitters of Different Geometries

Author:
Lotter, Carsten, Heiland, Josef J., Thurmann, Sebastian, Mauritz, Laura, Belder, Detlev
Source:
Analytical chemistry 2016 v.88 no.5 pp. 2856-2863
ISSN:
1520-6882
Subject:
glass, high performance liquid chromatography, mass spectrometry
Abstract:
We present and evaluate an approach for coupling liquid chromatography in glass chips with mass spectrometry via fully integrated electrospray emitters. We developed an instrumental platform which allows a robust and reproducible operation of high performance chip chromatography coupled to mass spectrometry. A comparison of differently shaped emitters, from flat over edged to pulled geometries, revealed that all types performed equally well for typical nano-HPLC flow rates. At very low flow rates below 50 nL·min–¹ very sharp, pulled nanospray emitters turned out to be mandatory for the generation of a stable electrospray.
Agid:
5304527