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Protein Binding Bends the Gold Nanoparticle Capped DNA Sequence: Toward Novel Energy-Transfer-Based Photoelectrochemical Protein Detection

Author:
Ma, Zheng-Yuan, Ruan, Yi-Fan, Xu, Fei, Zhao, Wei-Wei, Xu, Jing-Juan, Chen, Hong-Yuan
Source:
Analytical chemistry 2016 v.88 no.7 pp. 3864-3871
ISSN:
1520-6882
Subject:
DNA, binding proteins, biosensors, energy transfer, gold, nanogold, nucleotide sequences, quantum dots, transcription (genetics)
Abstract:
In this work, we present a novel energy-transfer (ET)-based photoelectrochemical (PEC) probing of DNA–protein interactions, which associates intimately with many important intracellular processes in transcriptional regulatory networks. Specifically, Au nanoparticles (NPs) were confined onto the CdS quantum dots (QDs) functionalized PEC surface by the formation of duplex DNA, the subsequent binding of the TATA binding protein (TBP) and the resulting distortion of the Au NPs capped DNA sequence could adjust the interparticle distance and thereby modulate the PEC performance of CdS QDs through the ET process between the CdS QDs and Au NPs. Using the duplex DNA sequence as a rigid spacer, the relationship between the photocurrent quenching effect and the spacing distance was also studied and some experimental conditions were optimized, on the basis of which a novel ET-based PEC TBP biosensor was realized with high sensitivity and selectivity.
Agid:
5304660