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Alkaloidal metabolites from Aspergillus felis and their activities against Paracoccidioides brasiliensis
- Mendes, Graziele, de Menezes Oliveira, Djalma, Kohlhoff, Markus, Rosa, Carlos Augusto, de Almeida Alves, Tânia Maria, Zani, Carlos Leomar, Rosa, Luiz Henrique, Johann, Susana, Barros Cota, Betania
- Phytochemistry letters 2016 v.17 pp. 28-35
- Aspergillus, Paracoccidioides brasiliensis, active ingredients, antifungal properties, etiological agents, fungi, methylene chloride, minimum inhibitory concentration, nuclear magnetic resonance spectroscopy, rocks, secondary metabolites, Chile
- In this study, the fungus Aspergillus felis (strain UFMGCB 8030) was isolated from rocks in the Atacama Desert, Chile. Its CH2Cl2 extract exhibited antifungal activity (MIC of 1.9μg/mL) against Paracoccidioides brasiliensis (Pb18), which is the etiological agent for paracoccidioidomycosis. The crude extract was purified, and a new cytochalasin derivative, cytochalasin Z15E (1), and one ardeemin derivative, 5-N-acetyl-8-β-isopropyl-ardeemin (4), in addition to five known secondary metabolites, rosellichalasin (2), cytochalasin E (3), gancidin (5), pseurotin A1 (6), and 2,4-dihydroxyacetophenone (7), were isolated. The structures of these compounds were confirmed by 1D and 2D NMR spectroscopy and HR-ESIMS. Cytochalasin E was the most active compound with MIC an value of 3.6μM, while the other isolated compounds exhibited weak antifungal activities (MIC values >100.0μM).