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Alkaloidal metabolites from Aspergillus felis and their activities against Paracoccidioides brasiliensis

Mendes, Graziele, de Menezes Oliveira, Djalma, Kohlhoff, Markus, Rosa, Carlos Augusto, de Almeida Alves, Tânia Maria, Zani, Carlos Leomar, Rosa, Luiz Henrique, Johann, Susana, Barros Cota, Betania
Phytochemistry letters 2016 v.17 pp. 28-35
Aspergillus, Paracoccidioides brasiliensis, active ingredients, antifungal properties, etiological agents, fungi, methylene chloride, minimum inhibitory concentration, nuclear magnetic resonance spectroscopy, rocks, secondary metabolites, Chile
In this study, the fungus Aspergillus felis (strain UFMGCB 8030) was isolated from rocks in the Atacama Desert, Chile. Its CH2Cl2 extract exhibited antifungal activity (MIC of 1.9μg/mL) against Paracoccidioides brasiliensis (Pb18), which is the etiological agent for paracoccidioidomycosis. The crude extract was purified, and a new cytochalasin derivative, cytochalasin Z15E (1), and one ardeemin derivative, 5-N-acetyl-8-β-isopropyl-ardeemin (4), in addition to five known secondary metabolites, rosellichalasin (2), cytochalasin E (3), gancidin (5), pseurotin A1 (6), and 2,4-dihydroxyacetophenone (7), were isolated. The structures of these compounds were confirmed by 1D and 2D NMR spectroscopy and HR-ESI⿿MS. Cytochalasin E was the most active compound with MIC an value of 3.6μM, while the other isolated compounds exhibited weak antifungal activities (MIC values >100.0μM).