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Proteomic characterization of hempseed (Cannabis sativa L.)

Aiello, Gilda, Fasoli, Elisa, Boschin, Giovanna, Lammi, Carmen, Zanoni, Chiara, Citterio, Attilio, Arnoldi, Anna
Journal of proteomics 2016 v.147 pp. 187-196
Arabidopsis thaliana, Cannabis sativa, amino acid composition, computer software, databases, digestibility, essential amino acids, foods, genes, polyacrylamide gel electrophoresis, protein synthesis, proteins, proteome, proteomics
This paper presents an investigation on hempseed proteome. The experimental approach, based on combinatorial peptide ligand libraries (CPLLs), SDS-PAGE separation, nLC-ESI-MS/MS identification, and database search, permitted identifying in total 181 expressed proteins. This very large number of identifications was achieved by searching in two databases: Cannabis sativa L. (56 gene products identified) and Arabidopsis thaliana (125 gene products identified). By performing a protein–protein association network analysis using the STRING software, it was possible to build the first interactomic map of all detected proteins, characterized by 137 nodes and 410 interactions. Finally, a Gene Ontology analysis of the identified species permitted to classify their molecular functions: the great majority is involved in the seed metabolic processes (41%), responses to stimulus (8%), and biological process (7%).Hempseed is an underexploited non-legume protein-rich seed. Although its protein is well known for its digestibility, essential amino acid composition, and useful techno-functional properties, a comprehensive proteome characterization is still lacking. The objective of this work was to fill this knowledge gap and provide information useful for a better exploitation of this seed in different food products.