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Proteomic characterization of hempseed (Cannabis sativa L.)
- Aiello, Gilda, Fasoli, Elisa, Boschin, Giovanna, Lammi, Carmen, Zanoni, Chiara, Citterio, Attilio, Arnoldi, Anna
- Journal of proteomics 2016 v.147 pp. 187-196
- Arabidopsis thaliana, Cannabis sativa, amino acid composition, computer software, databases, digestibility, essential amino acids, foods, genes, polyacrylamide gel electrophoresis, protein synthesis, proteins, proteome, proteomics
- This paper presents an investigation on hempseed proteome. The experimental approach, based on combinatorial peptide ligand libraries (CPLLs), SDS-PAGE separation, nLC-ESI-MS/MS identification, and database search, permitted identifying in total 181 expressed proteins. This very large number of identifications was achieved by searching in two databases: Cannabis sativa L. (56 gene products identified) and Arabidopsis thaliana (125 gene products identified). By performing a protein–protein association network analysis using the STRING software, it was possible to build the first interactomic map of all detected proteins, characterized by 137 nodes and 410 interactions. Finally, a Gene Ontology analysis of the identified species permitted to classify their molecular functions: the great majority is involved in the seed metabolic processes (41%), responses to stimulus (8%), and biological process (7%).Hempseed is an underexploited non-legume protein-rich seed. Although its protein is well known for its digestibility, essential amino acid composition, and useful techno-functional properties, a comprehensive proteome characterization is still lacking. The objective of this work was to fill this knowledge gap and provide information useful for a better exploitation of this seed in different food products.