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A Facile Method to Synthesize Histones with Posttranslational Modification Mimics

Author:
Wang, Zhiyong U., Wang, Yane-Shih, Pai, Pei-Jing, Russell, William K., Russell, David H., Liu, Wenshe R.
Source:
Biochemistry 2012 v.51 no.26 pp. 5232-5234
ISSN:
1520-4995
Subject:
acetylation, histones, lysine, methylation, phosphorylation, post-translational modification, serine, thiols
Abstract:
Using an evolved pyrrolysyl-tRNA synthetase-tRNAᴾʸˡ pair, a Se-alkylselenocysteine was genetically incorporated into histone H3 with a high protein expression yield. Quantitative oxidative elimination of Se-alkylselenocysteine followed by Michael addition reactions with various thiol nucleophiles generated biologically active mimics of H3 with posttranslational modifications including lysine methylation, lysine acetylation, and serine phosphorylation.
Agid:
5309773