U.S. flag

An official website of the United States government


Main content area

Assessment of volatile profile as potential marker of chilling injury of basil leaves during postharvest storage

Cozzolino, Rosaria, Pace, Bernardo, Cefola, Maria, Martignetti, Antonella, Stocchero, Matteo, Fratianni, Florinda, Nazzaro, Filomena, De Giulio, Beatrice
Food chemistry 2016 v.213 pp. 361-368
Ocimum basilicum, air, basil, chilling injury, cultivars, electrolytes, emissions, gas chromatography-mass spectrometry, headspace analysis, leaves, metabolites, principal component analysis, regression analysis, solid phase microextraction, storage temperature, volatile organic compounds
The volatile profile of three sweet basil cultivars, “Italico a foglia larga”, “Cammeo” and “Italiano classico”, packaged in air at 4 or 12°C until 9days, was monitored by solid phase microextraction with GC–MS. Chilling injury (CI) score and electrolyte leakage were also assessed. In total, 71 volatile organic compounds (VOCs) were identified in the headspace of basil samples. A preliminary principal component analysis highlighted the dominant effect of the cultivar on VOCs profiles. Data analysis by post-transformation of projection to latent structures regression (ptPLS2) clarified the role played by time and temperature of storage. Temperature influenced the emission of volatiles during storage, with much lower total volatile emissions at 4°C compared to 12°C. Finally, a ptPLS2 regression model performed on VOCs and the two CI parameters allowed selection of 10 metabolites inversely correlated to both CI parameters, which can be considered potential markers of CI in basil leaves.