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Comparative study of extraction efficiency and composition of protein recovered from chicken liver by acid–alkaline treatment

Author:
Xiong, Guoyuan, Gao, Xueqin, Wang, Peng, Xu, Xinglian, Zhou, Guanghong
Source:
Process biochemistry 2016 v.51 no.10 pp. 1629-1635
ISSN:
1359-5113
Subject:
acid treatment, actin, alkali treatment, chickens, humans, liver, myosin heavy chains, pH, polyacrylamide gel electrophoresis, protein composition, solubilization
Abstract:
This study focused on the extraction efficiency and composition of proteins recovered from chicken liver after extraction by acid/alkaline solubilization (pH 2.0, 2.5, 3.0 and 3.5) and (pH 10.5, 11.0, 11.5 and 12.0) and precipitation in pH 5.5. The highest extracted protein yield was obtained at pH values of 2.0, 11.5 and 12.0 (77.9%, 78.4% and 79.3% respectively), and the lowest yield (58.5%) was obtained at pH 3.5. Extraction of salt-soluble proteins was attributed to pH 3.0 and 3.5, whereas pH 10.5, 11.0 and 11.5 extracted more water-soluble proteins. Both acidic and alkaline solubilization extracted lots of insoluble proteins. The total pigment contents achieved by acid treatment were significantly higher (P<0.05) than those by alkaline treatment. The highest total pigment content was observed at pH 2.5. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) profiles indicated higher concentrations of myosin heavy chains (MHCs) and actin by alkaline extraction than acid treatment. In one word, this method of recovering valuable protein components by acid–alkaline treatment helps to produce innovative products and leads to an increased consumption of chicken liver by humans.
Agid:
5316854