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Preparation of guar gum scaffold film grafted with ethylenediamine and fish scale collagen, cross-linked with ceftazidime for wound healing application

Author:
Jana, Piyali, Mitra, Tapas, Selvaraj, Thirupathi Kumara Raja, Gnanamani, A., Kundu, P.P.
Source:
Carbohydrate polymers 2016 v.153 pp. 573-581
ISSN:
0144-8617
Subject:
Fourier transform infrared spectroscopy, Pseudomonas aeruginosa, Staphylococcus aureus, X-ray diffraction, biocompatibility, ceftazidime, collagen, crosslinking, drugs, guar gum, pH, scales (integument), scanning electron microscopy, tissue repair
Abstract:
Present study describes the synthesis of carboxymethyl guar gum (CMGG) from the native guar gum (GG) and the prepared CMGG is grafted with ethylenediamine (EDA) to form aminated CMGG. Then, fish scale collagen and aminated CMGG are cross-linked by ceftazidime drug through non- covalent ionic interaction. The resultant cross-linked film is subjected to the analysis of 1HNMR, ATR-FTIR, TGA, SEM and XRD. The TNBS results revealed that 45% of interaction between EDA and CMGG and 90–95% of Ceftazidime is released from aminated CMGG-Ceftazidime-Collagen (ACCC) film after 96h of incubation at physiological pH. In vitro cell line studies reveal the biocompatibility of the cross-linked film and the antimicrobial studies display the growth inhibition against Staphylococcus aureus and Pseudomonas aeruginosa organisms. Overall, the study indicates that the incorporation of Ceftazidime into collagen and aminated CMGG can improve the functional property of aminated CMGG as well as collagen, leading to its biomedical applications.
Agid:
5317862