PubAg

Main content area

Discrimination of Viable and Dead Fecal Bacteroidales Bacteria by Quantitative PCR with Propidium Monoazide

Author:
Bae, Sungwoo, Wuertz, Stefan
Source:
Applied and environmental microbiology 2009 v.75 no.9 pp. 2940-2944
ISSN:
0099-2240
Subject:
Bacteroides fragilis, DNA, bacteria, effluents, feces, polymerase chain reaction
Abstract:
Propidium monoazide (PMA) was optimized to discriminate between viable and dead Bacteroides fragilis cells and extracellular DNA at different concentrations of solids using quantitative PCR. Conditions of 100 μM PMA and a 10-min light exposure also excluded DNA from heat-treated cells of nonculturable Bacteroidales in human feces and wastewater influent and effluent.
Agid:
532069