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Discrimination of Viable and Dead Fecal Bacteroidales Bacteria by Quantitative PCR with Propidium Monoazide
- Bae, Sungwoo, Wuertz, Stefan
- Applied and environmental microbiology 2009 v.75 no.9 pp. 2940-2944
- Bacteroides fragilis, DNA, bacteria, effluents, feces, polymerase chain reaction
- Propidium monoazide (PMA) was optimized to discriminate between viable and dead Bacteroides fragilis cells and extracellular DNA at different concentrations of solids using quantitative PCR. Conditions of 100 μM PMA and a 10-min light exposure also excluded DNA from heat-treated cells of nonculturable Bacteroidales in human feces and wastewater influent and effluent.