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Natriuretic peptides stimulate oocyte meiotic resumption in bovine

Author:
Cesaro, Matheus P De, Macedo, Mariana P., Santos, Joabel T., Rosa, Paulo R.A, Ludke, Charles A., Rissi, Vitor B., Gasperin, Bernardo G., Gonçalves, Paulo B.D
Source:
Animal reproduction science 2015 v.159 pp. 52-59
ISSN:
0378-4320
Subject:
cattle, cyclic AMP, cyclic GMP, forskolin, gene expression, germinal vesicle, meiosis, messenger RNA, mice, models, natriuretic peptides, receptors, swine
Abstract:
The aim of the present study was to evaluate the expression of mRNA encoding natriuretic peptides (NPs) and their receptors in the cumulus-oocyte complex in cattle, a monovular mammalian species, and also to investigate the role of NPs in oocyte meiotic resumption in vitro. mRNA was observed for the NP precursor type-A (NPPA), type-C (NPPC), NP receptor-1 (NPR-1), receptor-2 (NPR-2) and receptor-3 (NPR-3) in bovine cumulus cells, and NPR-2 mRNA was observed in oocytes. These results are different from those obtained in mouse and pig models. The effects of NPPA, NP precursor type-B (NPPB) and NPPC on the resumption of arrested meiosis maintained by forskolin were studied at three different doses (10, 100 and 1000nM) with a 12h culture system. The germinal vesicle breakdown rates were greater (P≤0.05) in oocytes that were cultured in the presence of one or a combination of NPs (from 44% to 73%) than the negative control (from 24% to 27%). Additionally, it was demonstrated that the concentration of cyclic guanosine 3′,5′-monophosphate (cGMP) is increased by NPPA and NPPC in oocytes and cumulus cells after 3h of in vitro maturation. However, in both groups, the concentration of cyclic adenosine 3′,5′-monophosphate (cAMP) in the oocyte did not increase between 3 and 6h of culture, even when forskolin was used. In summary, we observed the presence of mRNA for NPs and their receptors in the bovine cumulus-oocyte complex and demonstrated that, in vitro, NPPA, NPPB and NPPC stimulate oocyte meiotic resumption in a monovular species.
Agid:
5325518