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Induction of settlement in larvae of the mussel Mytilus galloprovincialis using neuroactive compounds

Author:
Sánchez-Lazo, Clara, Martínez-Pita, Inés
Source:
Aquaculture 2012 v.344-349 pp. 210-215
ISSN:
0044-8486
Subject:
Mytilus galloprovincialis, acetylcholine, acute effects, epinephrine, gamma-aminobutyric acid, hatcheries, industry, ions, larvae, lethal concentration 50, mortality, mussels, potassium, potassium chloride, potassium sulfate, seawater, toxicity
Abstract:
We investigated the effect on Mytilus galloprovincialis larval settlement, as well as the toxicity, of serial concentrations in filtered seawater of acetylcholine (AC), γ-aminobutiric acid (GABA); 3-isobutyl-1-methylxanthine (IBMX); and the potassium ion in the form of potassium chloride (KCl) and potassium sulfate (K₂SO₄). All the substances assayed induced larval settlement and peak responses were above 90% in exposures to 10⁻²molL⁻¹ (M) AC, 10⁻⁴ and 10⁻⁵M epinephrine, 10⁻³M GABA and 20, 30 and 40mM KCl. The optimal concentration of K⁺ varied depending on the anionic component of the compound assayed, and peak settlement response to KCl was higher (100%) than that achieved with K₂SO₄ (69.7%). The estimated LC50 of the compounds assayed ranged from 9.4×10⁻⁶M (GABA) to 3.1×10⁻²M (KCl). GABA, IBMX and K₂SO₄ treatments displayed toxic effects in all the active concentrations. In contrast, AC 10⁻⁵ M, epinephrine 10⁻⁴ and 10⁻⁵M and KCl 20mM treatments enhanced larval settlement without an acute short-term effect on mortality. These results provide new insights on the molecular mechanisms controlling settlement in M. galloprovincialis larvae, and yield promising outcomes for the mussel industry to find a reliable method to enhance larval settlement in hatcheries.
Agid:
532571