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Photocontrolled Nanoparticles for On-Demand Release of Proteins

Author:
Azagarsamy, Malar A., Alge, Daniel L., Radhakrishnan, Srinidhi J., Tibbitt, Mark W., Anseth, Kristi S.
Source:
Biomacromolecules 2012 v.13 no.8 pp. 2219-2224
ISSN:
1526-4602
Subject:
chemical elements, encapsulation, fluorescent proteins, nanoparticles, polymerization, therapeutics
Abstract:
We describe here light-regulated swelling and degradation features of polymeric nanoparticles that are produced using an inverse microemulsion polymerization method. We demonstrate the phototriggered release characteristics of the nanoparticles by sequestering protein molecules and releasing them using light as a trigger. Furthermore, the intracellular translocation of the nanoparticles, along with its fluorescent protein payload, was achieved using a cell-penetrating peptide-based surface modification. We expect that the noncovalent encapsulation of proteins using nanoparticles and their photo triggered release using an external light would provide opportunities for achieving intracellular release of molecular therapeutics for on-demand requirements.
Agid:
5327341