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A novel exendin-4 human serum albumin fusion protein, E2HSA, with an extended half-life and good glucoregulatory effect in healthy rhesus monkeys

Author:
Zhang, Ling, Wang, Lin, Meng, Zhiyun, Gan, Hui, Gu, Ruolan, Wu, Zhuona, Gao, Lei, Zhu, Xiaoxia, Sun, Wenzhong, Li, Jian, Zheng, Ying, Dou, Guifang
Source:
Biochemical and biophysical research communications 2014 v.445 pp. 511-516
ISSN:
0006-291X
Subject:
Macaca mulatta, blood glucose, dipeptidyl-peptidase IV, dose response, excretion, fasting, food intake, glucagon-like peptide 1, glucose, glucose tolerance tests, half life, human serum albumin, hypoglycemic agents, insulin secretion, islets of Langerhans, medicinal properties, noninsulin-dependent diabetes mellitus, rats, secretin, therapeutics
Abstract:
Glucagon-like peptide-1 (GLP-1) has attracted considerable research interest in terms of the treatment of type 2 diabetes due to their multiple glucoregulatory functions. However, the short half-life, rapid inactivation by dipeptidyl peptidase-IV (DPP-IV) and excretion, limits the therapeutic potential of the native incretin hormone. Therefore, efforts are being made to develop the long-acting incretin mimetics via modifying its structure. Here we report a novel recombinant exendin-4 human serum albumin fusion protein E2HSA with HSA molecule extends their circulatory half-life in vivo while still retaining exendin-4 biological activity and therapeutic properties. In vitro comparisons of E2HSA and exendin-4 showed similar insulinotropic activity on rat pancreatic islets and GLP-1R-dependent biological activity on RIN-m5F cells, although E2HSA was less potent than exendin-4. E2HSA had a terminal elimation half-life of approximate 54h in healthy rhesus monkeys. Furthermore, E2HSA could reduce postprandial glucose excursion and control fasting glucose level, dose-dependent suppress food intake. Improvement in glucose-dependent insulin secretion and control serum glucose excursions were observed during hyperglycemic clamp test (18h) and oral glucose tolerance test (42h) respectively. Thus the improved physiological characterization of E2HSA make it a new potent anti-diabetic drug for type 2 diabetes therapy.
Agid:
5329712