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Identification of hepatitis B virus-specific CTL epitopes presented by HLA-A∗33:03 in peripheral blood mononuclear cells from patients and transgenic mice

Pan, Xuwen, Ding, Huiguo, Zhou, Xuyu, Tien, Po
Biochemical and biophysical research communications 2014 v.449 pp. 135-140
Hepatitis B virus, T-lymphocytes, cytotoxicity, epitopes, genotype, hepatitis B, liver, mice, patients, peptides, staining, transgenic animals, China
Cytotoxic T lymphocyte (CTL) epitopes in the HBV protein of hepatitis B virus (HBV) may play a key role in viral control and liver damage. The aim of this study was to identify and study the function of HLA-A∗33:03-restricted CTL epitopes in HBV protein of the HBV genotypes B and C, which are epidemic in China. Sixteen HBV peptides were predicated by computational analysis, and synthesized peptides were examined for their affinity to HLA-A∗33:03 using a stable cell line. After being analyzed by enzyme-linked immunospot and cytolytic activity assays, as well as the tetramers staining method using peripheral blood mononuclear cells isolated from HBV-infected patients, five peptides (Hbs245–253, HBs335–343, HBc119–127, HBc104–112, and HBp391–399) were chosen to further confirm their HLA_A∗33:03 restriction in transgenic mice.