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Anti-infective peptide IDR-1002 augments monocyte chemotaxis towards CCR5 chemokines

Madera, Laurence, Hancock, Robert E.W.
Biochemical and biophysical research communications 2015 v.464 pp. 800-806
CCR5 receptor, chemokine CCL3, chemokine CCL5, chemotaxis, immunomodulators, innate immunity, mitogen-activated protein kinase, monocytes, pathogens, peptides, phosphorylation
Innate defense regulator (IDR) peptides are a class of immunomodulators which enhance and modulate host innate immune responses against microbial pathogens. While IDR-mediated protection against a range of bacterial pathogens is dependent on enhanced monocyte recruitment to the site of infection, the mechanisms through which they increase monocyte trafficking remain unclear. In this study, anti-infective peptide IDR-1002 was shown to enhance monocyte chemotaxis towards chemokines CCL3 and CCL5. This enhancement correlated with the selective upregulation of CCR5 surface expression by peptide-treated monocytes. It was found that IDR-1002 enhancement of monocyte chemotaxis was fully dependent on CCR5 function. Furthermore, IDR-1002 enhanced chemokine-induced monocyte p38 MAPK phosphorylation in a CCR5-dependent fashion. Overall, these results indicate that peptide IDR-1002 can selectively influence monocyte recruitment by host chemokines through the regulation of chemokine receptors.