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Activation of farnesoid X receptor downregulates monocyte chemoattractant protein-1 in murine macrophage

Author:
Li, Liangpeng, Zhang, Qian, Peng, Jiahe, Jiang, Chanjui, Zhang, Yan, Shen, Lili, Dong, Jinyu, Wang, Yongchao, Jiang, Yu
Source:
Biochemical and biophysical research communications 2015 v.467 pp. 841-846
ISSN:
0006-291X
Subject:
bile acids, chemokine CCL2, chromatin, homeostasis, inflammation, lipids, luciferase, macrophages, messenger RNA, mice, precipitin tests, tissues
Abstract:
Farnesoid X receptor (FXR) is a member of the nuclear receptor superfamily, which plays important roles in bile acids/lipid homeostasis and inflammation. Monocyte chemoattractant protein-1 (MCP-1) contributes to macrophage infiltration into body tissues during inflammation. Here we investigated whether FXR can regulate MCP-1 expression in murine macrophage. FXR activation down regulate MCP-1 mRNA and protein levels in ANA-1 and Raw264.7 cells. Luciferase reporter assay, Gel shift and Chromatin immunoprecipitation assays have revealed that the activated FXR bind to the FXR element located in −738 bp ∼ −723 bp in MCP-1 promoter. These results suggested that FXR may serve as a novel target for regulating MCP-1 levels for the inflammation related diseases therapies.
Agid:
5332699