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Extended HSR/CARD domain mediates AIRE binding to DNA

Author:
Maslovskaja, Julia, Saare, Mario, Liiv, Ingrid, Rebane, Ana, Peterson, Pärt
Source:
Biochemical and biophysical research communications 2015 v.468 pp. 913-920
ISSN:
0006-291X
Subject:
caspases, staining, DNA, luciferase, amino acids, signal peptide, chromatin, introns, promoter regions
Abstract:
Autoimmune regulator (AIRE) activates the transcription of many genes in an unusual promiscuous and stochastic manner. The mechanism by which AIRE binds to the chromatin and DNA is not fully understood, and the regulatory elements that AIRE target genes possess are not delineated. In the current study, we demonstrate that AIRE activates the expression of transiently transfected luciferase reporters that lack defined promoter regions, as well as intron and poly(A) signal sequences. Our protein-DNA interaction experiments with mutated AIRE reveal that the intact homogeneously staining region/caspase recruitment domain (HSR/CARD) and amino acids R113 and K114 are key elements involved in AIRE binding to DNA.
Agid:
5332859