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Lipase-Catalyzed Dissipative Self-Assembly of a Thixotropic Peptide Bolaamphiphile Hydrogel for Human Umbilical Cord Stem-Cell Proliferation

Author:
Das, Apurba K., Maity, Indrajit, Parmar, Hamendra S., McDonald, Tom O., Konda, Maruthi
Source:
Biomacromolecules 2015 v.16 no.4 pp. 1157-1168
ISSN:
1526-4602
Subject:
carboxylic ester hydrolases, cell culture, cell viability, energy, humans, hydrogels, hydrolysis, hydroxyl radicals, nanofibers, stem cells, umbilical cord
Abstract:
We report lipase-catalyzed inclusion of p-hydroxy benzylalcohol to peptide bolaamphiphiles. The lipase-catalyzed reactions of peptide bolaamphiphiles with p-hydroxy benzylalcohol generate dynamic combinatorial libraries (DCL) in aqueous medium that mimic the natural dissipative system. The peptide bolaamphiphile 1 (HO-WY-Suc-YW–OH) reacts with p-hydroxy benzylalcohol in the presence of lipase forming an activated diester building block. The activated diester building block self-assembles to produce nanofibrillar thixotropic hydrogel. The subsequent hydrolysis results in the dissipation of energy to form nonassembling bolaamphiphile 1 with collapsed nanofibers. The thixotropic DCL hydrogel matrix is used for 3D cell culture experiments for different periods of time, significantly supporting the cell survival and proliferation of human umbilical cord mesenchymal stem cells.
Agid:
5333849