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Characterization of two thymosins as immune-related genes in common carp (Cyprinus carpio L.)

Xiao, Zhangang, Shen, Jing, Feng, Hong, Liu, Hong, Wang, Yaping, Huang, Rong, Guo, Qionglin
Developmental and comparative immunology 2015 v.50 no.1 pp. 29-37
Cyprinus carpio, Danio rerio, Spring viraemia of carp virus, T-lymphocytes, carp, complementary DNA, early development, gene expression regulation, gene overexpression, genes, immune response, messenger RNA, peptides, proteins, thymosin
Prothymosin alpha (ProTα) and thymosin beta (Tβ) belong to thymosin family, which consists of a series of highly conserved peptides involved in stimulating immune responses. ProTα b and Tβ are still poorly studied in teleost. Here, the full-length cDNAs of ProTα b and Tβ-like (Tβ-l) were cloned and identified in common carp (Cyprinus carpio L.). The expressions of carp ProTα b and Tβ-l exhibited rise-fall pattern and then trended to be stable during early development. After spring viraemia of carp virus (SVCV) infection, the carp ProTα b and Tβ-l transcripts were significantly up-regulated in some immune-related organs. When transiently over-expressed carp ProTα b and Tβ-l in zebrafish, these two proteins up-regulated the expressions of T lymphocytes-related genes (Rag 1, TCR-γ, CD4 and CD8α). These results suggest that carp ProTα b and Tβ may ultimately enhance the immune response during viral infection and modulate the development of T lymphocytes in teleost.