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Role of CD4+ and CD8α+ T cells in protective immunity against Edwardsiella tarda infection of ginbuna crucian carp, Carassius auratus langsdorfii

Yamasaki, Masatoshi, Araki, Kyosuke, Nakanishi, Teruyuki, Nakayasu, Chihaya, Yamamoto, Atsushi
Fish & shellfish immunology 2014 v.36 no.1 pp. 299-304
CD4-positive T-lymphocytes, Carassius auratus, Edwardsiella tarda, cell-mediated immunity, cytotoxic T-lymphocytes, cytotoxicity, fish, gene expression, gene expression regulation, interferon-gamma, pathogens, protective effect
Edwardsiella tarda is an intracellular pathogen that causes edwardsiellosis in fish. Our previous study suggests that cell-mediated immunity (CMI) plays an essential role in protection against E. tarda infection. In the present study, we adoptively transferred T-cell subsets sensitized with E. tarda to isogenic naïve ginbuna crucian carp to determination the T-cell subsets involved in protecting fish from E. tarda infection. Recipients of CD4+ and CD8α+ cells acquired significant resistance to infection with E. tarda 8 days after sensitization, indicating that helper T cells and cytotoxic T lymphocytes plays crucial roles in protective immunity to E. tarda. Moreover, transfer of sensitized CD8α+ cells up-regulated the expression of genes encoding interferon-γ (IFN-γ) and perforin, suggesting that protective immunity to E. tarda involves cell-mediated cytotoxicity and interferon-γ-mediated induction of CMI. The results establish that CMI plays a crucial role in immunity against E. tarda. These findings provide novel insights into understanding the role of CMI to intracellular pathogens of fish.