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DNA methylation is associated with expression level changes of galectin gene in mantle wound healing process of pearl oyster, Pinctada fucata

Author:
Li, Yaoguo, Huang, Xiande, Guan, Yunyan, Shi, Yu, Zhang, Hua, He, Maoxian
Source:
Fish & shellfish immunology 2015 v.45 no.2 pp. 912-918
ISSN:
1050-4648
Subject:
DNA methylation, Pinctada fucata, gene expression, gene expression regulation, genes, immune response, innate immunity, messenger RNA, oysters, promoter regions, proteins, quantitative polymerase chain reaction, tissue repair, tissues
Abstract:
Galectin is one important member of pattern recognition proteins that plays a pivotal role in regulating innate immune response of invertebrates. In this study, we cloned the promoter sequence of a tandem-repeat galectin from the pearl oyster Pinctada fucata (P. fucata). The quantitative real-time PCR analysis revealed that galectin mRNA expression in mantle tissues were firstly up-regulated from time points of 2 h–24 h, and then down-regulated from time points of 24 h–168 h after mantle injury. The genome methylation level of mantle tissue was inversely related to galectin mRNA expression (Pearson correlation: −0.554, P: 0.154). The “CpG4-6” methylation level in promoter region of galectin was significant positive correlated with the mRNA expression (Pearson correlation: 0.313, P: 0.049). The results indicated that galectin gene may be involved in immune response in mantle wound healing process of P. fucata, and DNA methylation may be a regulation factor of gene expression.
Agid:
5336360