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Comparison of Renal Biomarkers with Glomerular Filtration Rate in Susceptibility to the Detection of Gentamicin-Induced Acute Kidney Injury in Dogs
- Sasaki, A., Sasaki, Y., Iwama, R., Shimamura, S., Yabe, K., Takasuna, K., Ichijo, T., Furuhama, K., Satoh, H.
- Journal of comparative pathology 2014 v.151 no.2-3 pp. 264-270
- Beagle, biomarkers, blood serum, creatinine, dogs, epithelium, equations, gentamicin, glomerular filtration rate, kidneys, necrosis, subcutaneous injection, urea nitrogen
- Fourteen renal biomarkers were compared with measurement of glomerular filtration rate (GFR) in detecting acute kidney injury (AKI) in beagle dogs given gentamicin (40 mg/kg/day by subcutaneous injection) for 7 consecutive days. Serum and urinary biomarkers were measured before administration of gentamicin and then on days 4 and 8 after starting administration. GFR was derived by use of a simplified equation. Increased urinary cystatin C and decreased GFR occurred from day 4 and were detected before increases in blood urea nitrogen (BUN) and serum creatinine concentrations and changes in other urinary parameters. The closest correlation was between urinary cystatin C and GFR. At termination, microscopical examination revealed extensive necrosis of proximal tubular epithelium with hyaline casts in the kidney of treated dogs. These data indicate that urinary cystatin C is the most sensitive index of kidney injury and GFR reflects the kidney functional mass.