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Membrane-Bound Pyrroloquinoline Quinone-Dependent Dehydrogenase in Gluconobacter oxydans M5, Responsible for Production of 6-(2-Hydroxyethyl) Amino-6-Deoxy-L-Sorbose

Author:
Yang, Xue-Peng, Wei, Liu-Jing, Lin, Jin-Ping, Yin, Bo, Wei, Dong-Zhi
Source:
Applied and environmental microbiology 2008 v.74 no.16 pp. 5250-5253
ISSN:
0099-2240
Subject:
Gluconobacter oxydans, drugs, enzymes, gene targeting, oxidation, proteins
Abstract:
A membrane-bound protein purified from Gluconobacter oxydans M5 was confirmed to be a pyrroloquinoline quinone-dependent D-sorbitol dehydrogenase. Gene disruption and complementation experiments demonstrated that this enzyme is responsible for the oxidation of 1-(2-hydroxyethyl) amino-1-deoxy-D-sorbitol (1NSL) to 6-(2-hydroxyethyl) amino-6-deoxy-L-sorbose (6NSE), which is the precursor of an antidiabetic drug, miglitol.
Agid:
534006