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Effect of supplementation of multi‐microbe probiotic product on growth performance, apparent digestibility, cecal microbiota and small intestinal morphology of broilers

Author:
Kim, J. S., Ingale, S. L., Kim, Y. W., Kim, K. H., Sen, S., Ryu, M. H., Lohakare, J. D., Kwon, I. K., Chae, B. J.
Source:
Journal of animal physiology and animal nutrition 2012 v.96 no.4 pp. 618-626
ISSN:
0931-2439
Subject:
Bifidobacterium, Clostridium, antibiotics, body weight, chicks, coliform bacteria, crude protein, digestibility, duodenum, experimental diets, feed conversion, feed intake, growth performance, ileum, nutrients, probiotics, villi
Abstract:
The present study investigated the effect of inclusion of multi‐microbe probiotic product on growth performance, apparent digestibility of nutrients, cecal microbiota and small intestinal morphology in broilers. Four hundred days‐old Ross chicks were randomly allotted to five treatments on the basis of body weight (BW). Each treatment had four replicates of 20 chicks in each. Experimental diets were fed in two phases, starter (day 0–21) and finisher (day 22–35). Dietary treatments were; basal diet without any antimicrobial (NC), basal diet added with 20 mg Avilamycin/kg of diet (PC), 107 cfu multi‐microbe probiotic/kg of diet (P1), 108 cfu multi‐microbe probiotic/kg of diet (P2), and 109 cfu multi‐microbe probiotic/kg of diet (P3). Overall BW gain and feed conversion ratio were better (p < 0.05) for treatments PC, P2 and P3 compared with NC and P1, with P1 being better (p < 0.05) than NC. Overall feed intake in treatments PC, P1, P2 and P3 were greater (p < 0.05) than NC. Apparent digestibility of dry matter and crude protein were greater (p < 0.05) in treatments PC, P2 and P3 compared with NC, with P1 being intermediate and not different form NC, PC, P2 and P3. At d 21 and 35, treatments PC, P1, P2 and P3 showed lower (p < 0.05) cecal Clostridium and Coliforms count in relation to NC. Moreover, cecal Clostridium (d 21) and Coliforms (d 21 and 35) count were lower (p < 0.05) in treatment PC in relation to P1; with P2 and P3 being intermediate and not different from PC. However, there was no effect of dietary treatments on cecal total anaerobic bacteria and Bifidobacterium spp. count. The villus height of duodenum in treatment PC was greater (p < 0.05) than NC, with P1, P2 and P3 being intermediate. Villus height of ileum in treatment PC was greater (p < 0.05) than in treatments P1 and NC, whereas it remained comparable among treatments PC, P2 and P3. Villus height to crypt depth ratio of ileum was greater (p < 0.05) for treatment PC, P2 and P3 compared with that in P1 and NC. It is concluded that multi‐microbe probiotic inclusion at 108 and 109 cfu/kg diet had beneficial effects on broilers growth performance, apparent digestibility of nutrients and intestinal morphology and can be used as replacement to antibiotics growth promoter in broiler nutrition.
Agid:
534501