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Effective cutaneous vaccination using an inactivated chikungunya virus vaccine delivered by Foroderm

Author:
Rudd, Penny A., Raphael, Anthony P., Yamada, Miko, Nufer, Kaitlin L., Gardner, Joy, Le, Thuy T.T., Prow, Natalie A., Dang, Nhung, Schroder, Wayne A., Prow, Tarl W., Suhrbier, Andreas
Source:
Vaccine 2015 v.33 no.39 pp. 5172-5180
ISSN:
0264-410X
Subject:
Chikungunya virus, T-lymphocytes, antibodies, antigen-presenting cells, cytokines, lymph nodes, mice, silica, vaccination, vaccines, viremia
Abstract:
Foroderm is a new cutaneous delivery technology that uses high-aspect ratio, cylindrical silica microparticles, that are massaged into the skin using a 3D-printed microtextured applicator, in order to deliver payloads across the epidermis. Herein we show that this technology is effective for delivery of a non-adjuvanted, inactivated, whole-virus chikungunya virus vaccine in mice, with minimal post-vaccination skin reactions. A single topical Foroderm-based vaccination induced T cell, Th1 cytokine and antibody responses, which provided complete protection against viraemia and disease after challenge with chikungunya virus. Foroderm vaccination was shown to deliver fluorescent, virus-sized beads across the epidermis, with beads subsequently detected in draining lymph nodes. Foroderm vaccination also stimulated the egress of MHC II+ antigen presenting cells from the skin. Foroderm thus has potential as a simple, cheap, effective, generic, needle-free technology for topical delivery of vaccines.
Agid:
5351023