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Application of pH-sensitive fusogenic polymer-modified liposomes for development of mucosal vaccines

Author:
Watarai, Shinobu, Iwase, Tana, Tajima, Tomoko, Yuba, Eiji, Kono, Kenji, Sekiya, Yukio
Source:
Veterinary immunology and immunopathology 2014 v.158 no.1-2 pp. 62-72
ISSN:
0165-2427
Subject:
Salmonella Enteritidis, bacteria, blood serum, cecum, chickens, excretion, immune response, immunization, immunoglobulin G, interferon-gamma, interleukin-4, lymphocytes, messenger RNA, mice, polymers, protective effect, spleen, vaccines
Abstract:
To evaluate the usefulness of pH-sensitive fusogenic polymer (succinylated poly(glycidol) (SucPG) and 3-methylglutarylated poly(glycidol) (MGluPG))-modified liposomes as mucosal vaccine in the induction of a protective immune responses was evaluated. Mice were nasally immunized with OVA-containing SucPG-modified liposomes. After immunization, significant Ag-specific Abs were detected in the serum and intestine. When sera were analyzed for isotype distribution, antigen-specific IgG1 Ab responses were noted in mice immunized with OVA-containing polymer-unmodified liposomes, whereas immunization with OVA-containing SucPG-modified liposomes resulted in the induction of OVA-specific IgG1, IgG2a and IgG3 Ab responses. In spleen lymphocytes from mice immunized with OVA-containing SucPG-modified liposomes, both IFN-γ and IL-4 mRNA were detected. The same result was obtained also in the mouse immunized with OVA-containing MGluPG-modified liposomes. Furthermore, we examined the induction of immune responses in chickens following intraocular immunization with Salmonella Enteritidis Ag-containing MGluPG-modified liposomes, and the protective effect against the challenge with S. Enteritidis. Immunization with S. Enteritidis Ag-containing MGluPG-modified liposomes induced significant Ab responses against S. Enteritidis in the serum and intestine. Less fecal excretion of bacteria was observed in chickens immunized with S. Enteritidis Ag-containing MGluPG-modified liposomes after challenge. The numbers of bacteria in the caecum were also lower in immunized chickens than in unimmunized controls.
Agid:
5351313