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Class B CpG ODN stimulation upregulates expression of TLR21 and IFN-γ in chicken Harderian gland cells

Chrząstek, Klaudia, Borowska, Dominika, Kaiser, Pete, Vervelde, Lonneke
Veterinary immunology and immunopathology 2014 v.160 no.3-4 pp. 293-299
Toll-like receptors, adjuvants, chickens, gene expression, genes, interferon-gamma, interleukin-10, messenger RNA, oligodeoxyribonucleotides, respiratory tract diseases, vaccines, viruses
This study aimed to evaluate the response of Harderian gland (HG) cells after in vitro stimulation with class B synthetic oligodeoxyribonucleotides (ODN) containing CpG motifs. This knowledge is of importance for the development of mucosal vaccines for poultry, such as eye-drop or spray vaccines, to determine if class B CpG ODN can act as an vaccine adjuvant or as a prophylactic treatment mainly against respiratory disease viruses. The relative expression of Toll-like receptor 21 (TLR21), interferon (IFN)-γ, interleukin (IL)-1β and IL-10 genes were quantified at 1, 3, 6 and 18h post-stimulation of HG cells from 5-week-old birds. In addition, it was also investigated if expression of these genes was affected by the age of the birds (differences between 5- and 12-week-old birds), concentrations of ODN or cell preparation method used. Class B CpG ODN induced upregulation of TLR21 and IFN-γ mRNA expression levels at 1h post-stimulation depending on concentration of ODN used but only in HG cells isolated from young birds.