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In vitro effects of Eucalyptus staigeriana nanoemulsion on Haemonchus contortus and toxicity in rodents

Ribeiro, Wesley Lyeverton Correia, Camurça-Vasconcelos, Ana Lourdes Fernandes, Macedo, Iara Tersia Freitas, dos Santos, Jessica Maria Leite, de Araújo-Filho, José Vilemar, Ribeiro, Juliana de Carvalho, Pereira, Vanessa de Abreu, Viana, Daniel de Araújo, de Paula, Haroldo Cesar Beserra, Bevilaqua, Claudia Maria Leal
Veterinary parasitology 2015 v.212 no.3-4 pp. 444-447
Eucalyptus, Haemonchus contortus, acute toxicity, essential oils, gastrointestinal nematodes, hatching, hematologic tests, in vitro studies, larvae, larval development, larvicides, lethal dose 50, mice, nanoemulsions, nematicides, particle size, phytotherapy, rats, toxicity testing
Strategies for controlling gastrointestinal nematodes have been developed based on the use of numerous alternative methods, including the use of phytotherapy. New formulations of essential oils with anthelmintic activity have been proposed as a means to optimize their biological effects. Thus, the objective of this study was to formulate a nanoemulsion to optimize the nematicide effect of Eucalyptus staigeriana essential oil (EsEO). Initially, physico-chemical analyses were performed to verify the stability of the E. staigeriana nanoemulsion (EsNano). In vitro tests were conducted to evaluate the ovicidal and larvicidal activities of both EsNano and EsEO against Haemonchus contortus, and toxicology tests were then performed on rodents. The EsEO content in the nanoemulsion was 36.4% (v/v), and the mean particle size was 274.3nm. EsNano and EsEO inhibited larval hatching by 99% and 96.3% at 1 and 2mg/ml concentrations, respectively, and inhibited larval development by 96.3% and 97.3% at 8mg/ml concentrations. The acute toxicity test revealed that the EsNano and EsEO doses required to kill 50% of the mice (LD50) were 1,603.9 and 3,495.9mg/ml, respectively. EsNano did not alter the hematological parameters in the rats after treatment.