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Pulsed electric field and pH assisted selective extraction of intracellular components from microalgae Nannochloropsis
- Parniakov, Oleksii, Barba, Francisco J., Grimi, Nabil, Marchal, Luc, Jubeau, Sébastien, Lebovka, Nikolai, Vorobiev, Eugene
- Algal research 2015 v.8 pp. 128-134
- Nannochloropsis, antioxidant activity, carbohydrates, microalgae, pH, phenolic compounds, pigments, proteins, pulsed electric fields
- The study was aimed at investigation of the potential of pulsed electric field (PEF) pre-treatment as a preliminary step of pH-assisted aqueous extraction of algae components from microalgae Nannochloropsis suspensions. The PEF and sonication (S) were compared as pretreatment methods. They were applied at normal (pH=8.5) and basic (pH=11) conditions, and supplementary basic extraction (at pH=11) was done. The extracts were analyzed for content of pigments, proteins, carbohydrates, total phenolic compounds and antioxidant capacity. The colloidal stability of PEF- and S-pretreated suspensions was also evaluated. The data evidence that PEF technique allows selective extraction of a portion of pure proteins that are different from proteins extracted from S-pretreated suspensions. The discovered effects have shown the advantages of PEF-pretreatment at normal conditions (pH=8.5) and supplementary extraction at basic conditions (pH=11) for selective extraction of different intracellular components.