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Pulsed electric field and pH assisted selective extraction of intracellular components from microalgae Nannochloropsis

Parniakov, Oleksii, Barba, Francisco J., Grimi, Nabil, Marchal, Luc, Jubeau, Sébastien, Lebovka, Nikolai, Vorobiev, Eugene
Algal research 2015 v.8 pp. 128-134
Nannochloropsis, antioxidant activity, carbohydrates, microalgae, pH, phenolic compounds, pigments, proteins, pulsed electric fields
The study was aimed at investigation of the potential of pulsed electric field (PEF) pre-treatment as a preliminary step of pH-assisted aqueous extraction of algae components from microalgae Nannochloropsis suspensions. The PEF and sonication (S) were compared as pretreatment methods. They were applied at normal (pH=8.5) and basic (pH=11) conditions, and supplementary basic extraction (at pH=11) was done. The extracts were analyzed for content of pigments, proteins, carbohydrates, total phenolic compounds and antioxidant capacity. The colloidal stability of PEF- and S-pretreated suspensions was also evaluated. The data evidence that PEF technique allows selective extraction of a portion of pure proteins that are different from proteins extracted from S-pretreated suspensions. The discovered effects have shown the advantages of PEF-pretreatment at normal conditions (pH=8.5) and supplementary extraction at basic conditions (pH=11) for selective extraction of different intracellular components.