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Specific Pathways of Dietary Methylmercury and Inorganic Mercury Determined by Mercury Speciation and Isotopic Composition in Zebrafish (Danio rerio)

Author:
Feng, Caiyan, Pedrero, Zoyne, Gentès, Sophie, Barre, Julien, Renedo, Marina, Tessier, Emmanuel, Berail, Sylvain, Maury-Brachet, Régine, Mesmer-Dudons, Nathalie, Baudrimont, Magalie, Legeay, Alexia, Maurice, Laurence, Gonzalez, Patrice, Amouroux, David
Source:
Environmental Science & Technology 2015 v.49 no.21 pp. 12984-12993
ISSN:
1520-5851
Subject:
Danio rerio, bioaccumulation, biogeography, brain, diet, excretion, feces, fish, harvesting, isotope fractionation, liver, mercury, methylmercury compounds, models, muscles
Abstract:
An original approach is proposed to investigate inorganic (iHg) and methylmercury (MeHg) trophic transfer and fate in a model fish, Danio rerio, by combining natural isotopic fractionation and speciation. Animals were exposed to three different dietary conditions: (1) 50 ng Hg g–¹, 80% as MeHg; (2) diet enriched in MeHg 10 000 ng Hg g–¹, 95% as MeHg, and (3) diet enriched in iHg 10 000 ng Hg g–¹, 99% as iHg. Harvesting was carried out after 0, 7, 25, and 62 days. Time-dependent Hg species distribution and isotopic fractionation in fish organs (muscle, brain, liver) and feces, exhibited different patterns, as a consequence of their dissimilar metabolization. The rapid isotopic re-equilibration to the new MeHg-food source reflects its high bioaccumulation rate. Relevant aspects related to Hg excretion are also described. This study confirms Hg isotopic fractionation as a powerful tool to investigate biological processes, although its deconvolution and fully understanding is still a challenge.
Agid:
5362346