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Accelerated glycerol fermentation in Escherichia coli using methanogenic formate consumption

Author:
Richter, Katrin, Gescher, Johannes
Source:
Bioresource technology 2014 v.162 pp. 389-391
ISSN:
0960-8524
Subject:
Escherichia coli, Methanobacterium formicicum, coculture, ethanol, fermentation, formates, glycerol, methanogens, pH, succinic acid
Abstract:
Escherichia coli can ferment glycerol anaerobically only under very defined restrictive conditions. Hence, it was the aim of this study to overcome this limitation via a co-cultivation approach. Anaerobic glycerol fermentation by a pure E. coli culture was compared to a co-culture that also contained the formate-oxidizing methanogen Methanobacterium formicicum. Co-cultivation of the two strains led to a more than 11-fold increased glycerol consumption. Furthermore, it supported a constantly neutral pH and a shift from ethanol to succinate production. Moreover, M. formicicum was analyzed for its ability to grow on different standard media and a surprising versatility could be demonstrated.
Agid:
5364562