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Using recombinant cyanobacterium (Synechococcus elongatus) with increased carbohydrate productivity as feedstock for bioethanol production via separate hydrolysis and fermentation process
- Chow, Te-Jin, Su, Hsiang-Yen, Tsai, Tsung-Yu, Chou, Hsiang-Hui, Lee, Tse-Min, Chang, Jo-Shu
- Bioresource technology 2015 v.184 pp. 33-41
- Synechococcus elongatus, Synechococcus sp. PCC 7942, bioethanol, biomass, cell growth, cellulose, ethanol production, feedstocks, fermentation, genetic engineering, glucose, hydrolysates, hydrolysis, photosynthesis, sulfuric acid
- In this work, a recombinant cyanobacterium strain with increased photosynthesis rate, cell growth and carbohydrate production efficiency was genetically engineered by co-expressing ictB, ecaA, and acsAB (encoded for bacterial cellulose) in Synechococcus elongatus PCC7942. The resulting cyanobacterial biomass could be effectively hydrolyzed with dilute acid (2% sulfuric acid), achieving a nearly 90% glucose recovery at a biomass concentration of 80g/L. Bioethanol can be produced from fermenting the acidic hydrolysate of S. elongatus PCC7942 via separate hydrolysis and fermentation (SHF) process at a concentration of 7.2g/L and with a 91% theoretical yield.