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Physiological response of Clostridium ljungdahlii DSM 13528 of ethanol production under different fermentation conditions

Author:
Xie, Bin-Tao, Liu, Zi-Yong, Tian, Lei, Li, Fu-Li, Chen, Xiao-Hua
Source:
Bioresource technology 2015 v.177 pp. 302-307
ISSN:
0960-8524
Subject:
Clostridium ljungdahlii, acetates, acids, aldehyde oxidoreductases, calcium carbonate, cell growth, ethanol, ethanol production, fermentation, gene expression, genome, phase transition, physiological response, transcription (genetics)
Abstract:
In this study, cell growth, gene expression and ethanol production were monitored under different fermentation conditions. Like its heterotrophical ABE-producing relatives, a switch from acidogenesis to solventogenesis of Clostridium ljungdahlii during the autotrophic fermentation with CO/CO2 could be observed, which occurred surprisingly in the late-log phase rather than in the transition phase. The gene expression profiles indicated that aor1, one of the putative aldehyde oxidoreductases in its genome played a critical role in the formation of ethanol, and its transcription could be induced by external acids. Moreover, a low amount of CaCO3 was proved to have positive influences on the cell density and substrate utilization, followed by an increase of over 40% ethanol and 30% acetate formation.
Agid:
5365582