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Direct conversion of chicory flour into l(+)-lactic acid by the highly effective inulinase producer Lactobacillus paracasei DSM 23505

Author:
Petrova, Penka, Velikova, Petya, Popova, Luiza, Petrov, Kaloyan
Source:
Bioresource technology 2015 v.186 pp. 329-333
ISSN:
0960-8524
Subject:
Lactobacillus paracasei, batch fermentation, bioprocessing, biotechnology, cell walls, chicory, culture media, flour, fructose, glucose, inulin, inulinase, lactic acid, molecular weight, saccharification, sucrose
Abstract:
Highly effective bio-process for lactic acid (LA) production by simultaneous saccharification and fermentation (SSF) of chicory flour was developed. The strain used, Lactobacillus paracasei DSM 23505 produced natural inulinase (EC 3.2.1.80) with molecular weight ∼130kDa, located in the cell wall fraction. In batch fermentation with optimized medium content and fermentation conditions, a complete conversion of 136g/L chicory flour (89.3% inulin and 10.7% mix of sucrose, fructose and glucose) into 123.7g/L LA was achieved. These yield and conversion rate are the highest obtained by SSF for LA production from inulin. The high efficiency, the cheap fermentation broth and the simple process performance disclose the promising use of the chicory flour in industrial biotechnology for LA production.
Agid:
5366035