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Reducing β-glucosidase supplementation during cellulase recovery using engineered strain for successive lignocellulose bioconversion

Author:
Guo, Hong, Zou, Shaolan, Liu, Boshi, Su, Rongxin, Huang, Renliang, Qi, Wei, Zhang, Minhua, He, Zhimin
Source:
Bioresource technology 2015 v.187 pp. 362-368
ISSN:
0960-8524
Subject:
beta-glucosidase, biotransformation, cellulose, corn cobs, endo-1,4-beta-glucanase, ethanol, fermentation, genetically engineered microorganisms, lignocellulose
Abstract:
Enzyme recycling by re-adsorption is one of the primary methods for reducing enzyme usage in lignocellulose conversion. This work proposes the combination of an engineered yeast strain that expresses β-glucosidase with enzyme recycling to reduce the amount of supplemented β-glucosidase in enzyme recycling experiments. Using the engineered strain, a slight increase in ethanol concentration was obtained after a 96-h fermentation of pretreated corncobs. Ethanol concentrations increased by 34.7% and 62.7% in the following two recycle rounds using the engineered strain compared with those using its parental strain without β-glucosidase addition. Furthermore, with the addition of β-glucosidase at 30CBU/g cellulose, the ethanol concentration after two recycle rounds exceeded 90% of that observed in the first SSF round with the engineered strain at a high initial cellulase loading of 45FPU/g cellulose.
Agid:
5366063