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Effect of microalgae supplementation on the silage quality and anaerobic digestion performance of Manyflower silvergrass

Li, Lianhua, Sun, Yongming, Yuan, Zhenhong, Kong, Xiaoying, Wao, Yao, Yang, Ligui, Zhang, Yi, Li, Dong
Bioresource technology 2015 v.189 pp. 334-340
Enterococcus, Lactobacillus, ammonium nitrogen, anaerobic digestion, bacterial communities, community structure, feed quality, high-throughput nucleotide sequencing, lactic acid, methane, microalgae, pH, silage
The silage quality of Manyflower silvergrass with microalgae supplementation was investigated, and the variation in bacterial communities during ensilage period was analyzed by high-throughput sequencing technology. In addition, the specific methane yields of the silages were also evaluated. Results showed that the samples with microalgae supplementation have lower pH value and higher lactic acid concentration (8.0mg/gFM). Meanwhile, higher NH3-N concentration was observed since microalgae were N-rich material. Microalgae additions also influenced the bacterial community structure. For the samples without microalgae, the major genus was Enterococcus from day 1 to day 3, and then Lactobacillus became the dominant genus. While the prevalent genus represented in the samples with microalgae supplementation was Lactobacillus. The specific methane yields of all samples were within the range of 153±1mL/gVS to 178±11mL/gVS and no significant difference was observed.