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High yields of fatty acid and neutral lipid production from cassava bagasse hydrolysate (CBH) by heterotrophic Chlorella protothecoides

Author:
Chen, Junhui, Liu, Xiaoguang, Wei, Dong, Chen, Gu
Source:
Bioresource technology 2015 v.191 pp. 281-290
ISSN:
0960-8524
Subject:
Chlorella, algal oils, bagasse, batch fermentation, biodiesel, biomass production, cassava, endo-1,4-beta-glucanase, enzymatic hydrolysis, fatty acid composition, fatty acids, feedstocks, fuel production, hydrolysates, reducing sugars, triacylglycerols
Abstract:
The fermentation process for high yields of fatty acid and neutral lipid production from cassava bagasse hydrolysate (CBH) was developed by heterotrophic Chlorella protothecoides. An efficient single-step enzymatic hydrolysis of cassava bagasse (CB) by cellulase was firstly developed to produce >30g/L of reducing sugars. The concentrated CBH was subsequently applied in a batch culture, producing 7.9g/L of dry biomass with yield of 0.44g/g reducing sugar and 34.3wt% of fatty acids and 48.6wt% of neutral lipids. Furthermore, fed-batch fermentation using CBH achieved higher yields of fatty acids (41.0wt% and a titer of 5.83g/L) and neutral lipids (58.4wt% and yield of 0.22g/g reducing sugar). Additionally, the fatty acid profile analysis showed that the intercellular lipid was suitable to prepare high-quality biodiesel. This study demonstrated the feasibility of using CBH as low-cost feedstock to produce crude algal oil for sustainable biodiesel production.
Agid:
5366219