Jump to Main Content
Effects of glycerol on enzymatic hydrolysis and ethanol production using sugarcane bagasse pretreated by acidified glycerol solution
- Zhang, Zhanying, Wong, Heng H., Albertson, Peter L., Harrison, Mark D., Doherty, William O.S., O’Hara, Ian M.
- Bioresource technology 2015 v.192 pp. 367-373
- cellulases, enzymatic hydrolysis, ethanol, ethanol fermentation, ethanol production, glucans, glycerol, sugarcane bagasse
- In this study, for the first time the effects of glycerol on enzymatic hydrolysis and ethanol fermentation were investigated. Enzymatic hydrolysis was inhibited slightly with 2.0wt% glycerol, leading to reduction in glucan digestibility from 84.9% without glycerol to 82.9% (72h). With 5.0wt% and 10.0wt% glycerol, glucan digestibility was reduced by 4.5% and 11.0%, respectively. However, glycerol did not irreversibly inhibit cellulase enzymes. Ethanol fermentation was not affected by glycerol up to 5.0wt%, but was inhibited slightly at 10.0wt% glycerol, resulting in reduction in ethanol yield from 86.0% in the absence of glycerol to 83.7% (20h). Based on the results of laboratory and pilot-scale experiments, it was estimated that 0.142kg ethanol can be produced from 1.0kg dry bagasse (a glucan content of 38.0%) after pretreatment with acidified glycerol solution.