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Neutral red-mediated microbial electrosynthesis by Escherichia coli, Klebsiella pneumoniae, and Zymomonas mobilis

Author:
Harrington, Timothy D., Mohamed, Abdelrhman, Tran, Vi N., Biria, Saeid, Gargouri, Mahmoud, Park, Jeong-Jin, Gang, David R., Beyenal, Haluk
Source:
Bioresource technology 2015 v.195 pp. 57-65
ISSN:
0960-8524
Subject:
Escherichia coli, Klebsiella pneumoniae, Zymomonas mobilis, acetates, bacteria, ethanol, fermentation, lactic acid, metabolites, protein synthesis
Abstract:
The aim of this work was to compare the effects of electrosynthesis on different bacterial species. The effects of neutral red-mediated electrosynthesis on the metabolite profiles of three microorganisms: Escherichia coli, Klebsiella pneumoniae, and Zymomonas mobilis, were measured and compared and contrasted. A statistically comprehensive analysis of neutral red-mediated electrosynthesis is presented using the analysis of end-product profiles, current delivered, and changes in cellular protein expression. K. pneumoniae displayed the most dramatic response to electrosynthesis of the three bacteria, producing 93% more ethanol and 76% more lactate vs. control fermentation with no neutral red and no electron delivery. Z. mobilis showed no response to electrosynthesis except elevated acetate titers. Stoichiometric comparison showed that NAD+ reduction by neutral red could not account for changes in metabolites during electrosynthesis. Neutral red-mediated electrosynthesis was shown to have multifarious effects on the three species.
Agid:
5366338