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Neutral red-mediated microbial electrosynthesis by Escherichia coli, Klebsiella pneumoniae, and Zymomonas mobilis

Harrington, Timothy D., Mohamed, Abdelrhman, Tran, Vi N., Biria, Saeid, Gargouri, Mahmoud, Park, Jeong-Jin, Gang, David R., Beyenal, Haluk
Bioresource technology 2015 v.195 pp. 57-65
Escherichia coli, Klebsiella pneumoniae, Zymomonas mobilis, acetates, bacteria, ethanol, fermentation, lactic acid, metabolites, protein synthesis
The aim of this work was to compare the effects of electrosynthesis on different bacterial species. The effects of neutral red-mediated electrosynthesis on the metabolite profiles of three microorganisms: Escherichia coli, Klebsiella pneumoniae, and Zymomonas mobilis, were measured and compared and contrasted. A statistically comprehensive analysis of neutral red-mediated electrosynthesis is presented using the analysis of end-product profiles, current delivered, and changes in cellular protein expression. K. pneumoniae displayed the most dramatic response to electrosynthesis of the three bacteria, producing 93% more ethanol and 76% more lactate vs. control fermentation with no neutral red and no electron delivery. Z. mobilis showed no response to electrosynthesis except elevated acetate titers. Stoichiometric comparison showed that NAD+ reduction by neutral red could not account for changes in metabolites during electrosynthesis. Neutral red-mediated electrosynthesis was shown to have multifarious effects on the three species.