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Biohydrogen production by co-fermentation of crude glycerol and apple pomace hydrolysate using co-culture of Enterobacter aerogenes and Clostridium butyricum

Pachapur, Vinayak Laxman, Sarma, Saurabh Jyoti, Brar, Satinder Kaur, Le Bihan, Yann, Buelna, Gerardo, Verma, Mausam
Bioresource technology 2015 v.193 pp. 297-306
Clostridium butyricum, Enterobacter aerogenes, apple pomace, byproducts, carbon, coculture, ethanol, fermentation, glycerol, hydrogen production, hydrolysates, inoculum, response surface methodology, wastes
Co-substrate utilization of various wastes with complementary characteristics can provide a complete medium for higher hydrogen production. This study evaluated potential of apple pomace hydrolysate (APH) co-fermented with crude glycerol (CG) for increased H2 production and decreased by-products formation. The central composite design (CCD) along with response surface methodology (RSM) was used as tool for optimization and 15g/L of CG, 5g/L of APH and 15% (v/v) inoculum were found to be optimum to produce as high as 26.07±1.57mmol H2/L of medium. The p-value of 0.0017 indicated that APH at lower concentration had a significant effect on H2 production. By using CG as sole carbon source, reductive pathway of glycerol metabolism was favored with 19.46mmol H2/L. However, with APH, oxidative pathway was favored with higher H2 production (26.07±1.57mmol/L) and decrease in reduced by-products (1,3-propanediol and ethanol) formation. APH inclusion enhanced H2 production, and decreased substrate inhibition.