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Nitrogen-removal efficiency of a novel aerobic denitrifying bacterium, Pseudomonas stutzeri strain ZF31, isolated from a drinking-water reservoir

Author:
Huang, Tinglin, Guo, Lin, Zhang, Haihan, Su, Junfeng, Wen, Gang, Zhang, Kai
Source:
Bioresource technology 2015 v.196 pp. 209-216
ISSN:
0960-8524
Subject:
Pseudomonas stutzeri, bioremediation, carbon nitrogen ratio, denitrification, denitrifying bacteria, drinking water, genes, nitrate nitrogen, nitrites, nitrogen, nitrogen balance, pH, response surface methodology, temperature
Abstract:
An aerobic denitrifier, identified as Pseudomonas stutzeri strain ZF31, was isolated from the Zhoucun drinking-water reservoir. Strain ZF31 removed 97% of nitrate nitrogen after 16h, without nitrite accumulation. Sequence amplification indicated the presence of the denitrification genes napA, nirS, norB, and nosZ. Nitrogen balance analysis revealed that approximately 75% of the initial nitrogen was removed as gas products. Response surface methodology (RSM) experiments showed that maximum removal of total nitrogen (TN) occurred at pH 8.23, a C/N ratio of 6.68, temperature of 27.72°C, and with shaking at 54.15rpm. The TN removal rate at low C/N ratio (i.e., 3) and low temperature (i.e., 10°C) was 73.30% and 60.08%, respectively. These results suggest that strain ZF31 has potential applications for the bioremediation of slightly polluted drinking-water reservoirs.
Agid:
5366550