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85-kDa protein of Trypanosoma cruzi purified by affinity chromatography used in the multiple antigen binding assay (MABA) for the diagnosis of T. cruzi infection in a Venezuelan rural community

Berrizbeitia, Mariolga, Ward, Brian J., Bubis, José, Gottschalk, Marcelo, Aché, Alberto, Perdomo, Deisy, Medina, Rafael, Medina, Mehudy, Spencer, Lilian, Ndao, Momar
Parasitology research 2010 v.106 no.5 pp. 1127-1134
Chagas disease, Trypanosoma cruzi, affinity chromatography, antibody detection, antigens, disease vectors, enzyme-linked immunosorbent assay, proteins, rural communities
No ideal test exists for Chagas’ disease, and better diagnostic strategies are needed. We determined the diagnostic utility of an 85-kDa Trypanosoma cruzi protein in a multiple antigen binding assay (MABA). A standardized MABA test based on concentrated trypomastigote excretory–secretory antigen (TESA) and an 85-kDa purified protein showed 100% sensitivity and specificity. In field conditions, 6/66 individuals tested in a region not thought to be endemic (Rio Brito) were identified as seropositive for T. cruzi infection with our MABA test. In parallel, an enzyme-linked immunosorbent assay based on fixed epimastigotes detected 7/66 positives, which were independently confirmed. These data suggest that the 85-kDa and TESA proteins could be used in the MABA format as a complementary tool for the diagnosis of latent Chagas’ disease. High anti-T. cruzi antibody detection rates, poor knowledge of Chagas’ disease and its vector, and the demonstration of infected vectors in the study community all suggest a significant risk of reemergence of T. cruzi infection in this region of Venezuela.