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85-kDa protein of Trypanosoma cruzi purified by affinity chromatography used in the multiple antigen binding assay (MABA) for the diagnosis of T. cruzi infection in a Venezuelan rural community

Author:
Berrizbeitia, Mariolga, Ward, Brian J., Bubis, José, Gottschalk, Marcelo, Aché, Alberto, Perdomo, Deisy, Medina, Rafael, Medina, Mehudy, Spencer, Lilian, Ndao, Momar
Source:
Parasitology research 2010 v.106 no.5 pp. 1127-1134
ISSN:
0932-0113
Subject:
Chagas disease, Trypanosoma cruzi, affinity chromatography, antibody detection, antigens, disease vectors, enzyme-linked immunosorbent assay, proteins, rural communities
Abstract:
No ideal test exists for Chagas’ disease, and better diagnostic strategies are needed. We determined the diagnostic utility of an 85-kDa Trypanosoma cruzi protein in a multiple antigen binding assay (MABA). A standardized MABA test based on concentrated trypomastigote excretory–secretory antigen (TESA) and an 85-kDa purified protein showed 100% sensitivity and specificity. In field conditions, 6/66 individuals tested in a region not thought to be endemic (Rio Brito) were identified as seropositive for T. cruzi infection with our MABA test. In parallel, an enzyme-linked immunosorbent assay based on fixed epimastigotes detected 7/66 positives, which were independently confirmed. These data suggest that the 85-kDa and TESA proteins could be used in the MABA format as a complementary tool for the diagnosis of latent Chagas’ disease. High anti-T. cruzi antibody detection rates, poor knowledge of Chagas’ disease and its vector, and the demonstration of infected vectors in the study community all suggest a significant risk of reemergence of T. cruzi infection in this region of Venezuela.
Agid:
537156