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Rearing the soil arthropod Folsomia candida (Collembola: Isotomidae) on agar plates and estimating biomass by protein staining with Ponceau S

Author:
Li, Yang Grace, Fallon, Ann M.
Source:
Applied entomology and zoology 2016 v.51 no.3 pp. 489-494
ISSN:
0003-6862
Subject:
Folsomia candida, agar, biomass, charcoal, imidacloprid, median effective concentration, quantitative analysis, rearing, soil, soil arthropods, staining, toxicity, toxicity testing
Abstract:
Folsomia candida Willem (Collembola: Isotomidae) is a common soil arthropod used in standardized toxicological testing. We describe a simple method for rearing Collembola on agar plates and evaluating toxicity in the absence of charcoal, which has potential detoxifying effects. We developed a quantitative method for determining biomass by reversible protein staining of hexane-treated Collembola with Ponceau S. Protein staining has advantages over photometric methods for enumerating Collembola, and agar substrate provides a simple medium for testing toxicity of water-soluble substances independent of soil composition. Using these protocols, the 50 % effective concentration (EC₅₀) for the insecticide imidacloprid is ~0.2 µM, or 0.05 mg/kg agar substrate.
Agid:
5373362